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Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation
Author(s) -
Desbrosses-Fonrouge Anne-Garlonn,
Voigt Katrin,
Schröder Astrid,
Arrivault Stéphanie,
Thomine Sébastien,
Krämer Ute
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.046
Subject(s) - arabidopsis thaliana , arabidopsis , xenopus , microbiology and biotechnology , yeast , mutant , chemistry , rna interference , green fluorescent protein , biology , biochemistry , rna , gene
The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1 D94A mutant or water‐injected oocytes. An AtMTP1‐GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter‐GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference‐mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.