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ASF/SF2 and SC35 regulate the glutamate receptor subunit 2 alternative flip/flop splicing
Author(s) -
Crovato Trine Elkjær,
Egebjerg Jan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.044
Subject(s) - exon , flip , rna splicing , alternative splicing , protein subunit , splice , enhancer , sr protein , biology , microbiology and biotechnology , genetics , gene , gene expression , rna , apoptosis
The properties of the glutamate receptor subunits 1–4 (GluR1–4) are influenced by the alternative splicing of two homologous and mutually exclusive exons flip and flop. The flip form is most abundant during early development, while the flop form is dominant in adults. From transfections with a GluR2 mini‐gene we show that flip is the preferred splice form in all tested cell lines, but coexpression of the SR‐proteins ASF/SF2 and SC35 increases the flop to flip splice ratio. The increased flop incorporation depends on ASF/SF2‐ and SC35‐dependent enhancer elements located in the flop exon, which stimulate the splicing between the flop exon and the preceding exon 13.