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Regulation of lipopolysaccharide‐induced inflammatory response by glutathione S‐transferase P1 in RAW264.7 cells
Author(s) -
Xue Bin,
Wu Yifan,
Yin Zhimin,
Zhang Hong,
Sun Suwen,
Yi Tangsheng,
Luo Lan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.034
Subject(s) - lipopolysaccharide , tumor necrosis factor alpha , p38 mitogen activated protein kinases , gstp1 , mapk/erk pathway , nitric oxide , transfection , kinase , chemistry , glutathione , microbiology and biotechnology , biology , biochemistry , endocrinology , enzyme , gene
Glutathione S‐transferase P1(GSTP1) plays an important role in the detoxification and xenobiotics metabolism. Here, we show that GSTP1 is also involved in LPS (lipopolysaccharide)‐induced inflammatory response. GSTP1 expression, determined at the transcription and translation levels, were upregulated by the LPS stimulation in RAW264.7 macrophage‐like cells. GSTP1 inhibited LPS‐induced mitogen‐activated protein kinases MAPKs including ERK, JNK and p38 as well as NF‐κB activation dose‐ and time‐dependently in transient transfected and stable transfected cells. Moreover this inhibition of the signaling pathways resulted in the decrease of tumor necrosis factor alpha (TNF‐α) and nitric oxide (NO) synthesis. These data suggest that the GSTP1 prevents LPS‐induced excessive production of pro‐inflammatory factors and plays an anti‐inflammatory role in response to LPS.