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Platelet activating factor induces cytoskeletal reorganization through Rho family pathway in THP‐1 macrophages
Author(s) -
Sumita Chinuyo,
Yamane Masako,
Matsuda Takahisa,
Maeda Makiko,
Nariai Tetsuro,
Fujio Yasushi,
Azuma Junichi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.06.022
Subject(s) - rhoa , cdc42 , microbiology and biotechnology , cytoskeleton , platelet activating factor , filopodia , rac1 , rac gtp binding proteins , chemistry , gtpase , signal transduction , biology , actin , cell , immunology , biochemistry
In the process of atherosclerosis, platelet activating factor (PAF) promotes the infiltration of inflammatory cells into atherosclerotic plaque by modulating their cytoskeleton. Here, we examined whether Rho family proteins are involved in PAF‐induced cytoskeletal reorganization in THP‐1 macrophages. PAF stimulation rapidly induced cell elongation, accompanied by filopodia formation. The inhibition of Rho family proteins by the overexpression of Rho‐GDI attenuated the PAF‐mediated morphological changes. Both RhoA and Cdc42 were activated in response to PAF. Inhibition of RhoA or Cdc42 by dominant negative mutants abrogated morphological changes induced by PAF. Collectively, PAF regulates cytoarchitecture through Rho family proteins in macrophages.