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The yeast osmosensitive mutant fps1 Δ transformed by the cauliflower BobTIP1;1 aquaporin withstand a hypo‐osmotic shock
Author(s) -
Prudent Sébastien,
Marty Francis,
Charbonnier Maryse
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.05.076
Subject(s) - aquaporin , osmotic shock , yeast , saccharomyces cerevisiae , osmoregulation , osmosis , mutant , biology , osmotic concentration , microbiology and biotechnology , water transport , membrane , biochemistry , gene , water flow , ecology , salinity , environmental engineering , engineering
Osmoregulation plays an important role in cellular responses to osmotic stress in plants and in yeast. Aquaporins contribute to osmotic adjustment by facilitating transport of water or solutes across membranes. The tonoplastic water channel BobTIP1;1 (original name BobTIP26‐1) genes are upregulated during dessication stress in cauliflower meristematic tissue. To investigate the physiological importance of BobTIP1;1, we expressed it in a Saccharomyces cerevisiae osmosensitive mutant fps1 Δ. We showed that the defect in the yeast glycerol plasma membrane transporter is complemented by a plant cDNA encoding the aquaporin BobTIP1;1 which is localized in the vacuolar membrane of the complemented yeast cells. To our knowledge, this is the first example of a plant aquaporin for which localization in the vacuolar membrane of yeast cells is related to an osmoresistant phenotype under hypo‐osmotic shock.