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Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress
Author(s) -
Fujikake Nobuhiro,
Nagai Yoshitaka,
Popiel H. Akiko,
Kano Hiroki,
Yamaguchi Masamitsu,
Toda Tatsushi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.05.074
Subject(s) - hsf1 , heat shock factor , gene isoform , rna splicing , heat shock , alternative splicing , microbiology and biotechnology , splicing factor , heat shock protein , transcription factor , messenger rna , transcription (linguistics) , biology , gene , genetics , rna , hsp70 , linguistics , philosophy
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock proteins in response to various types of stress. Although HSF1 activity is regulated by its post‐translational modifications, alterations in mRNA expression have also been suggested. We here identified three new alternatively spliced isoforms of Drosophila HSF (dHSF) mRNA, named dHSFb, dHSFc, and dHSFd. We found that the ratio of dHSFb increases upon heat exposure, while that of dHSFd increases upon cold exposure. The dHSFc and dHSFd isoforms showed greater transcriptional activity than the other isoforms. Our findings suggest that alternative splicing regulates the transcriptional activity of dHSF.