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Translational repression by the cis ‐acting element of structure‐anchored repression (CAESAR) of human ctgf / ccn2 mRNA
Author(s) -
Kubota Satoshi,
Mukudai Yoshiki,
Moritani Norifumi H.,
Nakao Kyouji,
Kawata Kazumi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.05.068
Subject(s) - psychological repression , ctgf , messenger rna , untranslated region , rna , translation (biology) , microbiology and biotechnology , gene expression , biology , gene , genetics , growth factor , receptor
The cis ‐acting element of structure‐anchored repression (CAESAR) is a post‐transcriptional regulatory element of gene expression, which is located in the 3′‐untranslated region (UTR) of the human ccn2 gene ( ctgf / ccn2 ). In this report, the repression mechanism of CAESAR, as well as the structural requirement, was investigated. Removal of minor stem‐loops from CAESAR resulted in proportional attenuation of the repressive function, whereas removal of the single bulge or modification of primary nucleotide sequence did not affect its functionality. In light of functional mechanism, CAESAR exerted no significant effects on stability or nuclear export of the cis ‐linked mRNA. However, this element significantly interfered with the association of such mRNA on ribosome and slowed down the translation process thereafter in vitro. A translation repression mechanism by RNA secondary structure to determine the basal ctgf / ccn2 expression level was uncovered herein.