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DNA‐binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles
Author(s) -
Krause Kirsten,
Kilbienski Isabell,
Mulisch Maria,
Rödiger Anja,
Schäfer Anke,
Krupinska Karin
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.05.059
Subject(s) - arabidopsis , plastid , arabidopsis thaliana , organelle , biology , mitochondrion , fusion protein , gene , microbiology and biotechnology , green fluorescent protein , mitochondrial dna , homology (biology) , genetics , chloroplast , mutant , recombinant dna
Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer‐based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functionality of these sequences was demonstrated by in vitro import assays into isolated organelles. Transient expression of GFP fusion proteins in protoplasts and onion epidermal cells confirmed the localisation of these proteins in plastids or mitochondria, respectively. The possession of organellar targeting sequences seems to be conserved among Why proteins of higher plant species, including potato p24.