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Expression and functional characterization of LRRC4 , a novel brain‐specific member of the LRR superfamily
Author(s) -
Zhang Qiuhong,
Wang Jieru,
Fan Songqing,
Wang Lili,
Cao Li,
Tang Ke,
Peng Cong,
Li Zheng,
Li Weifang,
Gan Kai,
Liu Zhongqi,
Li Xiaoling,
Shen Shourong,
Li Guiyuan
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.05.058
Subject(s) - carcinogenesis , cell cycle , biology , cell growth , central nervous system , microbiology and biotechnology , gene expression , gene , cancer research , genetics , neuroscience
LRRC4, a novel member of LRR superfamily thought to be involved in development and tumorigenesis of the nervous tissue, has the potential to suppress tumorigenesis and cell proliferation of U251MG cells. This study aimed at revealing the correlation between expression of LRRC4 and the maintenance of normal function and tumorigenesis suppression within the central nervous system. We systematically analyzed the expression and tissue distributions of the gene in tissues. Results showed that LRRC4 expression was limited to normal adult brain, both in human and in mouse, and exhibited a development‐regulated pattern, but was down‐regulated in brain tumor tissues and U251MG cell line. Furthermore, dynamic alterations in gene expression associated with cell cycle progression were investigated by using Tet‐on system. Results showed that LRRC4 induced a cell cycle delay at the late G1 phase, probably through the alteration of the expression of different cell cycle regulating proteins responsible for mediating G1‐S progression, such as p21 Waf1/Cip1 and p27 Kip1 , Cdk2 and PCNA, p‐ERK1/2. These findings suggest that LRRC4 may play an important role in maintaining normal function and suppressing tumorigenesis in the central nervous system.