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Specific inhibition of HIV‐1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis
Author(s) -
Li Zhaoyang,
Xiong Yong,
Peng Yu,
Pan Ji‧an,
Chen Yu,
Wu Xiaoyun,
Hussain Snawar,
Tien Po,
Guo Deyin
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.074
Subject(s) - small hairpin rna , rna interference , provirus , small interfering rna , biology , rna , microbiology and biotechnology , viral replication , virology , cancer research , virus , genetics , gene , genome
RNA interference (RNAi), a sequence‐specific RNA degradation mechanism mediated by small interfering RNA (siRNA), can be used not only as a research tool but also as a therapeutic strategy for viral infection. We demonstrated that intracellular expression of short hairpin RNA (shRNA) targeting human cyclin T1 (hCycT1), a cellular factor essential for transcription of messenger and genomic RNAs from the long terminal repeat promoter of provirus of human immunodeficiency virus type 1 (HIV‐1), could effectively suppress the replication of HIV‐1. We also showed that downregulation of hCycT1 did not cause apoptotic cell death, therefore, targeting cellular factor hCycT1 by shRNAs may provide an attractive approach for genetic therapy of HIV‐1 infection in the future.