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Compensatory regulation among ER chaperones in C. elegans
Author(s) -
Kapulkin Vadim,
Hiester Brian G.,
Link Christopher D.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.062
Subject(s) - caenorhabditis elegans , rna interference , gene knockdown , downregulation and upregulation , microbiology and biotechnology , biology , transgene , unfolded protein response , gene , green fluorescent protein , reporter gene , chaperone (clinical) , rna , genetics , gene expression , medicine , pathology
We have identified a particularly clear case of compensatory transcriptional regulation among ER chaperones in Caenorhabditis elegans using a GFP reporter transgene that is under the control of the promoter of hsp‐4 , a C. elegans homolog of GRP‐78/BiP. Knockdown by RNA interference of 9 known or predicted ER chaperones induced hsp‐4 upregulation via the ire‐1/xbp‐1 signaling cascade employed in the unfolded protein response. We show that this compensatory regulation is specific for ER chaperones, not dependent on RNA interference, and required for maintaining viability in worms containing a deletion of the hsp‐3 gene.