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Heterogeneities in EGF receptor density at the cell surface can lead to concave up scatchard plot of EGF binding
Author(s) -
Mayawala Kapil,
Vlachos Dionisios G.,
Edwards Jeremy S.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.059
Subject(s) - scatchard plot , epidermal growth factor , cooperative binding , dimer , receptor , binding site , biophysics , epidermal growth factor receptor , chemistry , cell surface receptor , plot (graphics) , microbiology and biotechnology , biochemistry , biology , mathematics , organic chemistry , statistics
The mechanism responsible for the concave up nature of the Scatchard plot of epidermal growth factor (EGF) binding on EGF receptor (EGFR) has been a controversial issue for more than a decade. Past efforts to mechanistically simulate the concave up nature of the Scatchard plot of EGF binding have shown that negative cooperativity in EGF binding on an EGFR dimer or inclusion of some external site or binding event can describe this behavior. However, herein we show that heterogeneity in the density of EGFR due to localization in certain regions of the plasma membrane, which has been experimentally reported, can also lead to concave up shape of the Scatchard plot of the EGF binding on EGFR.