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An NMR‐based docking model for the physiological transient complex between cytochrome f and cytochrome c 6
Author(s) -
Díaz-Moreno Irene,
Díaz-Quintana Antonio,
Ubbink Marcellus,
De la Rosa Miguel A.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.031
Subject(s) - plastocyanin , cytochrome , cytochrome f , docking (animal) , chemistry , stereochemistry , cytochrome c , cytochrome b6f complex , crystallography , biophysics , biochemistry , cytochrome b , biology , enzyme , photosystem ii , photosystem i , medicine , nursing , mitochondrion , gene , photosynthesis , mitochondrial dna
The physiological transient complex between cytochrome f (C f ) and cytochrome c 6 (C c 6 ) from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by NMR spectroscopy. The binding constant at low ionic strength is 8 ± 2 mM −1 , and the binding site of C c 6 for C f is localized around its exposed haem edge. On the basis of the experimental data, the resulting docking simulations suggest that C c 6 binds to C f in a fashion that is analogous to that of plastocyanin but differs between prokaryotes and eukaryotes.