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Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde
Author(s) -
Lim Eng-Kiat,
Jackson Rosamond G.,
Bowles Dianna J.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.016
Subject(s) - glycosyltransferase , arabidopsis thaliana , coniferyl alcohol , chemistry , biochemistry , arabidopsis , aldehyde , lignin , enzyme , mutant , gene , catalysis , organic chemistry
This study describes the substrate recognition profile of UGT72E1, an UDP–glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols.