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Sp1‐dependent regulation of Myeloid Elf‐1 like factor in human epithelial cells
Author(s) -
Koga Tomoaki,
Suico Mary Ann,
Nakamura Hideaki,
Taura Manabu,
Lu Zhuo,
Shuto Tsuyoshi,
Okiyoneda Tsukasa,
Kai Hirofumi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.015
Subject(s) - promoter , biology , microbiology and biotechnology , gene , rna interference , chemistry , gene expression , genetics , rna
Myeloid Elf‐1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony‐stimulating factor, interleukin‐3, lysozyme, human beta defensin‐2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5′‐flanking region of human MEF gene and identified a TATA‐less promoter region at −204/−54 which contains 4 putative binding sites for Sp1, two of which are essential in up‐regulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene.