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N 6 ‐Acetyladenosine: A new modified nucleoside from Methanopyrus kandleri tRNA
Author(s) -
Sauerwald Anselm,
Sitaramaiah Devarasetty,
McCloskey James A.,
Söll Dieter,
Crain Pamela F.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.014
Subject(s) - methanogen , crenarchaeota , biology , transfer rna , nucleoside , archaea , methanomicrobiales , thermophile , rna , population , biochemistry , genetics , enzyme , gene , bacteria , demography , methanosarcina , sociology
Post‐transcriptionally modified nucleosides are constituents of transfer RNA (tRNA) that are known to influence tertiary structure, stability and coding properties. Modifications in unfractionated tRNA from the phylogenetically unique archaeal methanogen Methanopyrus kandleri (optimal growth temperature 98 °C) were studied using liquid chromatography‐mass spectrometry to establish the extent to which they might differ from those of other methanogens. The exceptionally diverse population of nucleosides included four new nucleosides of unknown structure, and one that was characterized as N 6 ‐acetyladenosine, a new RNA constituent. The nucleoside modification pattern in M. kandleri tRNA is notably different from that of other archaeal methanogens, and is closer to that of the thermophilic crenarchaeota.