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Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum
Author(s) -
Sakurai Masayuki,
Ohtsuki Takashi,
Suzuki Tsutomu,
Watanabe Kimitsuna
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.009
Subject(s) - ascaris suum , nematode , ascaris , chemistry , mitochondrion , biochemistry , biology , zoology , helminths , ecology
To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post‐transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two‐codon families ending with a purine were analyzed. 5‐Carboxymethylaminomethyluridine (cmnm 5 U) was identified at the wobble positions of tRNA Lys , tRNA Glu and tRNA Gln , while 5‐carboxymethylaminomethyl‐2‐thiouridine (cmnm 5 s 2 U) was present intRNA UAA Leu and tRNA Trp. In most bacterial and mitochondrial tRNAs, the 2‐thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm 5 s 2 U is used intRNA UAA Leu and tRNA Trp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.