cda1 + , encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe

In Schizosaccharomyces pombe , a major role of chitin is to build up a complete spore. Here, we analyzed the cda1 + gene (SPAC19G12.03), which encodes a protein homologous to chitin deacetylases, to know whether it is required for spore formation in S. pombe . The homothallic Δ cda1 strain constructed by homologous recombination was found to form a little amount of abnormal spores that contained one, two, or three asci, similar to (but not as strong as) the phenotype observed in a deletion mutant of chs1 encoding chitin synthase 1. This phenotype is reversed by expression of S. cerevisiae chitin deacetylase CDA1 or CDA2 , suggesting that cda1 encodes a chitin deacetylase. To support the role of Cda1 in sporulation, the timing of expression of cda1 + mRNA increased during sporulation process. We also found that the Cda1 protein self‐associated when its binding was tested both by two‐hybrid system and immunoprecipitation. Thus, these data indicated that cda1 + is required for proper spore formation in S. pombe .