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cda1 + , encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe
Author(s) -
Matsuo Yasuhiro,
Tanaka Katsunori,
Matsuda Hideyuki,
Kawamukai Makoto
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.008
Subject(s) - schizosaccharomyces pombe , schizosaccharomyces , chitin , mutant , saccharomyces cerevisiae , biology , spore , chitin synthase , gene , homologous recombination , genetics , biochemistry , microbiology and biotechnology , chemistry , chitosan
In Schizosaccharomyces pombe , a major role of chitin is to build up a complete spore. Here, we analyzed the cda1 + gene (SPAC19G12.03), which encodes a protein homologous to chitin deacetylases, to know whether it is required for spore formation in S. pombe . The homothallic Δ cda1 strain constructed by homologous recombination was found to form a little amount of abnormal spores that contained one, two, or three asci, similar to (but not as strong as) the phenotype observed in a deletion mutant of chs1 encoding chitin synthase 1. This phenotype is reversed by expression of S. cerevisiae chitin deacetylase CDA1 or CDA2 , suggesting that cda1 encodes a chitin deacetylase. To support the role of Cda1 in sporulation, the timing of expression of cda1 + mRNA increased during sporulation process. We also found that the Cda1 protein self‐associated when its binding was tested both by two‐hybrid system and immunoprecipitation. Thus, these data indicated that cda1 + is required for proper spore formation in S. pombe .