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Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation
Author(s) -
Bellik Lydia,
Ledda Fabrizio,
Parenti Astrid
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.04.003
Subject(s) - progenitor cell , phenotype , peripheral blood mononuclear cell , progenitor , microbiology and biotechnology , biology , lineage (genetic) , endothelial stem cell , cellular differentiation , lineage markers , immunology , in vitro , stem cell , genetics , gene
The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties.