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Interleukin‐2‐dependent regulation of Na/K pump in human lymphocytes
Author(s) -
Marakhova Irina,
Karitskaya Inna,
Aksenov Nikolay,
Zenin Valerii,
Vinogradova Tatjana
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.096
Subject(s) - ouabain , downregulation and upregulation , chemistry , atpase , protein subunit , microbiology and biotechnology , protein expression , cell , biology , sodium , biochemistry , enzyme , gene , organic chemistry
The present study provides the first evidence that the abundance of catalytic α1‐subunit of Na,K‐ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti‐proliferative doses diminished the induction of α1 protein in activated lymphocytes. Furthermore, in competent T cells, IL‐2 increases both the transport activity of Na/K pump and the content of Na,K‐ATPase α1 protein in a time‐dependent manner. A correlation was found between the long‐term elevation in ouabain‐sensitive Rb influxes and the increase in α1 protein content in late activated T cells. These results suggest that (1) the increased expression of Na,K‐ATPase proteins underlie the cell cycle‐dependent upregulation of ion pump during T cell transformation, and (2) IL‐2 is involved in the regulated expression of Na,K‐ATPase in human lymphocytes.