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Quantitative aspects of endocytic activity in lipid‐mediated transfections
Author(s) -
Prasad Tekkatte Krishnamurthy,
Rangaraj Nandini,
Rao Nalam Madhusudhana
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.083
Subject(s) - transfection , endocytic cycle , endocytosis , microbiology and biotechnology , cell , cell cycle , mitosis , interphase , cationic liposome , chemistry , wortmannin , biology , biochemistry , signal transduction , gene , pi3k/akt/mtor pathway
Variation in transfection efficiency observed in different cell‐types is poorly understood. To investigate the influence of endocytic activity on lipid‐mediated transfections, we have monitored both the processes in 12 different cell‐types. The endocytic activity shows a strong positive correlation ( P < 0.01), with transfection efficiency. Treatment with wortmannin resulted in cell‐type‐dependent inhibition of transfection. Studies on M‐phase cells by confocal microscopy show that compared to interphase cells, uptake of cationic liposomes was substantially reduced. In addition, transfection efficiency of cells in mitotic phase was inhibited by >70% compared to controls. Our study based on several cell‐types demonstrates for the first time that quantitative aspects of endocytosis have decisive influence on the overall process of lipid‐mediated transgene expression.