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Induction of IGFBP‐1 expression by amino acid deprivation of HepG2 human hepatoma cells involves both a transcriptional activation and an mRNA stabilization due to its 3′UTR
Author(s) -
Averous Julien,
Maurin Anne-Catherine,
Bruhat Alain,
Jousse Céline,
Arliguie Céline,
Fafournoux Pierre
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.077
Subject(s) - messenger rna , amino acid , untranslated region , three prime untranslated region , downregulation and upregulation , chemistry , gene expression , microbiology and biotechnology , biology , biochemistry , gene
A dramatic overexpression of IGFBP‐1 is responsible for growth inhibition, in response to a low‐protein diet feeding. It has been demonstrated that a fall in the amino acid concentration was directly responsible for IGFBP‐1 induction. In this report, we sought to determine the mechanism by which amino acid limitation upregulates IGFBP‐1 expression. Our results show that both transcriptional activation and mRNA stabilization are involved. We also demonstrate that (i) the mGCN2/ATF4 pathway is not involved in this regulation and (ii) the 3′UTR of IGFBP‐1 mRNA is responsible for its destabilization and regulates its stability in response to amino acid starvation.