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Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium
Author(s) -
MacFadyen John R.,
Haworth Oliver,
Roberston David,
Hardie Deborah,
Webster Marie-Therese,
Morris Howard R.,
Panico Maria,
Sutton-Smith Mark,
Dell Anne,
van der Geer Peter,
Wienke Dirk,
Buckley Christopher D.,
Isacke Clare M.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.071
Subject(s) - fibroblast , stromal cell , monoclonal antibody , endothelium , microbiology and biotechnology , pericyte , biology , glycoprotein , antibody , endothelial stem cell , cell culture , cancer research , immunology , genetics , in vitro
Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.