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Origin, distribution and 3D‐modeling of Gr‐EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis
Author(s) -
Kudla Urszula,
Qin Ling,
Milac Adina,
Kielak Anna,
Maissen Cyril,
Overmars Hein,
Popeijus Herman,
Roze Erwin,
Petrescu Andrei,
Smant Geert,
Bakker Jaap,
Helder Johannes
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.047
Subject(s) - globodera rostochiensis , biology , potato cyst nematode , nematode , expansin , globodera pallida , botany , actinobacteria , gene , genetics , solanaceae , ecology , 16s ribosomal rna , gene expression
Southern analysis showed that Gr‐EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis , is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr‐EXPB1 domain 1 (D1) has a flat β‐barrel structure with surface‐exposed aromatic rings, whereas the 3D structure of Gr‐EXPB1‐D2 was remarkably similar to plant expansins. Gr‐EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil‐inhabiting Actinobacteria, and includes a bacterial type II carbohydrate‐binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer.