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The yeast eIF4E‐associated protein Eap1p attenuates GCN4 translation upon TOR‐inactivation
Author(s) -
Matsuo Ryu,
Kubota Hiroyuki,
Obata Tohru,
Kito Keiji,
Ota Kazuhisa,
Kitazono Takanari,
Ibayashi Setsuro,
Sasaki Takuma,
Iida Mitsuo,
Ito Takashi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.043
Subject(s) - eif4e , crosstalk , translation (biology) , microbiology and biotechnology , protein biosynthesis , eukaryotic translation , yeast , biology , tor signaling , initiation factor , amino acid , biochemistry , transcription factor , signal transduction , chemistry , gene , messenger rna , optics , physics
Amino acid‐starved yeast activates the eIF2α kinase Gcn2p to suppress general translation and to selectively derepress the transcription factor Gcn4p, which induces various biosynthetic genes to elicit general amino acid control (GAAC). Well‐fed yeast activates the target of rapamycin (TOR) to stimulate translation via the eIF4F complex. A crosstalk was demonstrated between the pathways for GAAC and TOR signaling: the TOR‐specific inhibitor rapamycin activates Gcn2p. Here we demonstrate that, upon TOR‐inactivation, the putative TOR‐regulated eIF4E‐associated protein Eap1p likely functions downstream of Gcn2p to attenuate GCN4 translation via a mechanism independent of eIF4E‐binding, thereby constituting another interface between the two pathways.