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Requirement of the transmembrane semaphorin Sema4C for myogenic differentiation
Author(s) -
Ko Ji-Ae,
Gondo Toshikazu,
Inagaki Shinobu,
Inui Makoto
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.03.022
Subject(s) - semaphorin , c2c12 , myogenesis , biology , transmembrane protein , microbiology and biotechnology , myocyte , biochemistry , receptor
Semaphorins constitute a large family of signaling proteins that contribute to axonal guidance. Here we demonstrate that the transmembrane semaphorin Sema4C is up‐regulated both in the early stage of differentiation of C2C12 mouse skeletal myoblasts into myotubes and during injury‐induced muscle regeneration in vivo. Depletion of Sema4C in C2C12 cells resulted in marked attenuation of myotube formation. A fusion protein containing the extracellular Sema domain and a peptide corresponding to the intracellular COOH‐terminal region of Sema4C each inhibited the differentiation of C2C12 cells. These findings indicate that Sema4C‐mediated interaction among myoblasts plays an important role in terminal myogenic differentiation.