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BLG‐e1 – A novel regulatory element in the distal region of the β‐lactoglobulin gene promoter
Author(s) -
Reichenstein Moshe,
German Tania,
Barash Itamar
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.061
Subject(s) - regulatory sequence , promoter , biology , microbiology and biotechnology , gene , gene expression , regulation of gene expression , exon , reporter gene , histone , genetics
β‐Lactoglobulin (BLG) is a major ruminant milk protein. A regulatory element, termed BLG‐e1, was defined in the distal region of the ovine BLG gene promoter. This 299‐bp element lacks the established cis ‐regulatory sequences that affect milk‐protein gene expression. Nevertheless, it alters the binding of downstream BLG sequences to histone H4 and the sensitivity of the histone–DNA complexes to trichostatin A treatment. In mammary cells cultured under favorable lactogenic conditions, BLG‐e1 acts as a potent, position‐independent silencer of BLG/luciferase expression, and similarly affects the promoter activity of the mouse whey acidic protein gene. Intragenic sequences upstream of BLG exon 2 reverse the silencing effect of BLG‐e1 in vitro and in transgenic mice.