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The low energy emitting states of the Lhca4 subunit of higher plant photosystem I
Author(s) -
Zucchelli Giuseppe,
Morosinotto Tomas,
Garlaschi Flavio M.,
Bassi Roberto,
Jennings Robert C.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.057
Subject(s) - excited state , fluorescence , photosystem i , emission spectrum , photosystem ii , excitation , dipole , anisotropy , quenching (fluorescence) , atomic physics , chemistry , molecular physics , physics , photosynthesis , spectral line , optics , biochemistry , organic chemistry , quantum mechanics , astronomy
The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non‐equilibrium distribution, leading to enhanced emission from the directly excited low‐energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735 nm (F713 and F735) and unusual spectral properties, have been identified. Both high‐energy states are populated when selective excitation is into the F735 state and the fluorescence anisotropy spectrum attains the value of 0.3 in the wavelength region where both emission states are present. This indicates that the two states are on the same Lhca4 complex and have transition dipoles with similar orientation.