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SKN1 , a novel plant defensin‐sensitivity gene in Saccharomyces cerevisiae , is implicated in sphingolipid biosynthesis
Author(s) -
Thevissen Karin,
Idkowiak-Baldys Jola,
Im Yang-Ju,
Takemoto Jon,
François Isabelle E.J.A.,
Ferket Kathelijne K.A.,
Aerts An M.,
Meert Els M.K.,
Winderickx Joris,
Roosen Johnny,
Cammue Bruno P.A.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.043
Subject(s) - sphingolipid , biosynthesis , saccharomyces cerevisiae , mutant , biology , biochemistry , gene , wild type
The antifungal plant defensin DmAMP1 interacts with the fungal sphingolipid mannosyl diinositolphosphoryl ceramide (M(IP) 2 C) and induces fungal growth inhibition. We have identified SKN1 , besides the M(IP) 2 C‐biosynthesis gene IPT1 , as a novel DmAMP1‐sensitivity gene in Saccharomyces cerevisiae . SKN1 was previously shown to be a KRE6 homologue, which is involved in β‐1,6‐glucan biosynthesis. We demonstrate that a Δ skn1 mutant lacks M(IP) 2 C. Interestingly, overexpression of either IPT1 or SKN1 complemented the skn1 mutation, conferred sensitivity to DmAMP1, and resulted in M(IP) 2 C levels comparable to the wild type. These results show that SKN1 , together with IPT1 , is involved in sphingolipid biosynthesis in S. cerevisiae .