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Similarities and differences in the transcriptional regulation of the leptin gene promoter in gastric and adipose cells
Author(s) -
Goı¨ot Hélène,
Laigneau Jean-Pierre,
Devaud Hélène,
Sobhani Iradj,
Bado André
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.031
Subject(s) - leptin , adipose tissue , gene , promoter , biology , endocrinology , microbiology and biotechnology , medicine , gene expression , genetics , obesity
The stomach was reported to synthesize and secrete leptin mainly in the gastric lumen. Gastric leptin release is markedly increased after food intake, by vagal cholinergic stimulation and by cholecystokinin and secretin. Here we show that human gastric MKN‐74 cells produce leptin that increases upon challenge with cholecystokinin, insulin, glucocorticoids and all‐trans retinoic acid through activation of the leptin gene promoter. In addition, we demonstrate that forskolin and BRL37344 which increased cAMP levels, fail to affect the activity of leptin gene promoter in MKN74 expressing β 3 ‐adrenoceptor cells but, induce a 2‐fold decrease in this activity in adipose 3T3‐L1 cells. These data described for the first time, similarities and more interestingly, differences in the regulation of the leptin gene promoter in gastric cells as compared to adipocytes.