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Genomic organization, promoter characterization and roles of Sp1 and AP‐2 in the basal transcription of mouse PDIP1 gene
Author(s) -
Zhou Jianlin,
Fan Changzheng,
Zhong Yingli,
Liu Yunhai,
Liu Mingjun,
Zhou Aidong,
Ren Kaiqun,
Zhang Jian
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.027
Subject(s) - caat box , promoter , tata box , gene , transcription (linguistics) , cpg site , biology , microbiology and biotechnology , exon , rna polymerase ii , start codon , genetics , general transcription factor , gene expression , messenger rna , dna methylation , linguistics , philosophy
The mouse polymerase delta‐interacting protein 1 gene, PDIP1 , is mapped to chromosome 7F3 region, spans approximately 16.7 kb, and is organized into six exons. The transcription start site (TSS) was determined to be G, corresponding to position of 162‐bp upstream of the translation start codon. The promoter region was found to lack TATA box or CCAAT box, instead, a CpG island was detected surrounding TSS. The region from −162 to +114 is required for basal transcriptional regulation of mouse PDIP1 gene, contains two AP‐2 and two Sp1 binding sites. The Sp1 site upstream of TSS activates, while the other Sp1 site and two AP‐2 sites suppress the transcription activity of mouse PDIP1 gene.