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Study of stem cell function using microarray experiments
Author(s) -
Perez-Iratxeta Carolina,
Palidwor Gareth,
Porter Christopher J.,
Sanche Neal A.,
Huska Matthew R.,
Suomela Brian P.,
Muro Enrique M.,
Krzyzanowski Paul M.,
Hughes Evan,
Campbell Pearl A.,
Rudnicki Michael A.,
Andrade Miguel A.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.02.020
Subject(s) - microarray , dna microarray , stem cell , microarray databases , computational biology , microarray analysis techniques , biology , function (biology) , microbiology and biotechnology , gene , gene expression , genetics
DNA Microarrays are used to simultaneously measure the levels of thousands of mRNAs in a sample. We illustrate here that a collection of such measurements in different cell types and states is a sound source of functional predictions, provided the microarray experiments are analogous and the cell samples are appropriately diverse. We have used this approach to study stem cells, whose identity and mechanisms of control are not well understood, generating Affymetrix microarray data from more than 200 samples, including stem cells and their derivatives, from human and mouse. The data can be accessed online (StemBase; http://www.scgp.ca:8080/StemBase/).