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Docking and fusion of insulin secretory granules in SUR1 knock out mouse β‐cells observed by total internal reflection fluorescence microscopy
Author(s) -
Kikuta Toshiteru,
Ohara-Imaizumi Mica,
Nakazaki Mitsuhiro,
Nishiwaki Chiyono,
Nakamichi Yoko,
Tei Chuwa,
Aguilar-Bryan Lydia,
Bryan Joseph,
Nagamatsu Shinya
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.01.074
Subject(s) - exocytosis , total internal reflection fluorescence microscope , fluorescence microscope , chemistry , lipid bilayer fusion , microbiology and biotechnology , biophysics , insulin , docking (animal) , cytoplasm , fusion , microscopy , membrane , biochemistry , biology , fluorescence , endocrinology , medicine , pathology , physics , linguistics , nursing , philosophy , quantum mechanics
To explore how the sulfonylurea receptor (SUR1) is involved in docking and fusion of insulin granules, dynamic motion of single insulin secretory granules near the plasma membrane was examined in SUR1 knock‐out (Sur1KO) β‐cells by total internal reflection fluorescence microscopy. Sur1KO β‐cells exhibited a marked reduction in the number of fusion events from previously docked granules. However, the number of docked granules declined during stimulation as a consequence of the release of docked granules into the cytoplasm vs. fusion with the plasma membrane. Thus, the impaired docking and fusion results in decreased insulin exocytosis from Sur1KO β‐cells.