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Cell‐cycle‐dependent regulation of the human and mouse Tome‐1 promoters
Author(s) -
Yoshida Kenichi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.01.055
Subject(s) - promoter , cell cycle , mitosis , biology , microbiology and biotechnology , transcription (linguistics) , luciferase , response element , repressor , transcription factor , tata box , gene , transfection , genetics , gene expression , linguistics , philosophy
Tome‐1, which refers to a trigger of mitotic entry 1, mediates the destruction of the mitosis‐inhibitory kinase, Wee1, via the E3 ligase, SCF. In turn, Tome‐1 itself is targeted for degradation by APC in the G1 phase of the cell cycle. In the present study, we analyzed the human and mouse Tome‐1 promoter regions. Using synchronized cultures of NIH3T3 cells transfected with Tome‐1 promoter/luciferase constructs, we showed that the promoter activity of Tome‐1 is activated at the G2/M phase. Using various Tome‐1 promoter/luciferase constructs, we showed that the CCAAT box located upstream of the transcription initiation site is important for the basal promoter activity. We identified a repressor element (cell‐cycle‐dependent element/cell cycle gene homology region) in the vicinity of the transcription start site, and mutations within this element diminished the cell‐cycle‐dependent transcriptional regulation of Tome‐1.