Premium
Inhibition of human telomerase by oligonucleotide chimeras, composed of an antisense moiety and a chemically modified homo‐oligonucleotide
Author(s) -
Tarkanyi Ilona,
Horváth András,
Szatmari Istvan,
Eizert Helga,
Vámosi György,
Damjanovich Sándor,
Ségal-Bendirdjian Evelyne,
Aradi Janos
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.01.041
Subject(s) - telomerase , oligonucleotide , reverse transcriptase , microbiology and biotechnology , telomerase reverse transcriptase , telomere , rna , moiety , biology , telomerase rna component , chimera (genetics) , chemistry , dna , biochemistry , stereochemistry , gene
Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s 4 dU) n moiety at its 3′ or 5′‐end. The increase of the thiolated chain length enhances the telomerase inhibitory potential, but decreases specificity, indicated by HIV reverse transcriptase inhibition. Chimeras with 5′ (s 4 dU) n s were more potent inhibitors than the antisense alone or the 3′ modified ones. Cy5‐labeled (s 4 dU) 4 AS and (s 4 dU) 8 AS proved the internalization of the oligonucleotides, raising the possibility to be tested as cellular anti‐telomerase agents.