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Auxin‐induced reactive oxygen species production requires the activation of phosphatidylinositol 3‐kinase
Author(s) -
Joo Jung Hee,
Yoo Ho Jung,
Hwang Inhwan,
Lee June Seung,
Nam Kyoung Hee,
Bae Yun Soo
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.01.018
Subject(s) - auxin , phosphatidylinositol , gravitropism , microbiology and biotechnology , reactive oxygen species , ly294002 , chemistry , kinase , biochemistry , arabidopsis , biology , mutant , gene
We recently reported that production of reactive oxygen species (ROS) is essential for auxin‐induced gravitropic signaling. Here, we investigated the role of phosphatidylinositol 3‐kinase and its product, PtdIns(3)P, in auxin‐mediated ROS production and the root gravitropic response. Pretreatment with LY294002, an inhibitor of PtdIns 3‐kinase activity, blocked auxin‐mediated ROS generation, and reduced the sensitivity of root tissue to gravistimulation. The amount of PtdIns(3)P increased in response to auxin, and this effect was abolished by pretreatment with LY294002. In addition, sequestration of PtdIns(3)P by transient expression of the endosome binding domain in protoplasts abrogated IAA‐induced ROS accumulation. These results indicate that activation of PtdIns 3‐kinase and its product PtdIns(3)P are required for auxin‐induced production of ROS and root gravitropism.