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Zizimin2: a novel, DOCK180‐related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes
Author(s) -
Nishikimi Akihiko,
Meller Nahum,
Uekawa Natsuko,
Isobe Ken-ichi,
Schwartz Martin A.,
Maruyama Mitsuo
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2005.01.006
Subject(s) - guanine nucleotide exchange factor , cdc42 , gtpase , subfamily , guanine , biology , cloning (programming) , gene , microbiology and biotechnology , nucleotide , ras superfamily , genetics , biochemistry , gtp' , enzyme , computer science , programming language
A novel superfamily of guanine nucleotide exchange factors for Rho GTPases includes DOCK180 and zizimin1. The zizimin subfamily includes three genes of which only zizimin1 has been cloned. We report here the cloning of zizimin2, identified in a screen for genes enriched in germinal center B cells. Zizimin2 and zizimin1 have similar primary structures and both proteins bound and activated Cdc42 but not the Cdc42‐related proteins TC10 or TCL. Their tissue distributions are distinct, however, with zizimin2 expressed predominantly in lymphocytes and an opposite pattern for zizimin1. Zizimin3 was also analyzed and showed distinct GTPase specificity and tissue distribution.