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DLC‐1, a Rho GTPase‐activating protein with tumor suppressor function, is essential for embryonic development
Author(s) -
Durkin Marian E.,
Avner Miriam R.,
Huh Chang-Goo,
Yuan Bao-Zhu,
Thorgeirsson Snorri S.,
Popescu Nicholas C.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.12.090
Subject(s) - microbiology and biotechnology , biology , carcinogenesis , embryonic stem cell , actin , suppressor , embryo , mutant , gtpase , small gtpase , neural tube , embryogenesis , gene , genetics , signal transduction
DLC‐1 (deleted in liver cancer 1) is a Rho GTPase‐activating protein that is able to inhibit cell growth and suppress tumorigenesis. We have used homologous recombination to inactivate the mouse DLC‐1 gene ( Arhgap7 ). Mice heterozygous for the targeted allele were phenotypically normal, but homozygous mutant embryos did not survive beyond 10.5 days post coitum. Histological analysis revealed that DLC‐1 −/− embryos had defects in the neural tube, brain, heart, and placenta. Cultured fibroblasts from DLC‐1‐deficient embryos displayed alterations in the organization of actin filaments and focal adhesions.