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Chrysin suppresses lipopolysaccharide‐induced cyclooxygenase‐2 expression through the inhibition of nuclear factor for IL‐6 (NF‐IL6) DNA‐binding activity
Author(s) -
Woo Kyung Jin,
Jeong Yong-Jin,
Inoue Hiroyasu,
Park Jong-Wook,
Kwon Taeg Kyu
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.12.048
Subject(s) - chrysin , chemistry , luciferase , downregulation and upregulation , lipopolysaccharide , propolis , electrophoretic mobility shift assay , pharmacology , biochemistry , microbiology and biotechnology , gene expression , biology , immunology , transfection , gene , antioxidant , flavonoid , food science
Chrysin is a natural, biologically active compound extracted from many plants, honey and propolis. It possesses potent anti‐inflammation, anti‐cancer and anti‐oxidation properties. The mechanism by which chrysin suppresses COX‐2 expression remains poorly understood. In the present report, we investigated the effect of chrysin on the expression of COX‐2 in lipopolysaccharide (LPS)‐activated Raw 264.7 cells. Chrysin significantly suppressed the LPS‐induced COX‐2 protein and mRNA expression in a dose‐dependent manner. The ability of chrysin to suppress the expression of the COX‐2 was investigated using luciferase reporters controlled by various cis ‐elements in COX‐2 promoter region. Mutational analysis and electrophoretic mobility shift assay verified that nuclear factor for IL‐6 was identified as responsible for the chrysin‐mediated COX‐2 downregulation. These results will provide new insights into the anti‐inflammatory and anti‐carcinogenic properties of chrysin.