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HCO 3 ‐ ‐independent rescue from apoptosis by stilbene derivatives in rat cardiomyocytes
Author(s) -
Tanabe Shigeru,
Wang Xiaoming,
Takahashi Nobuyuki,
Uramoto Hiromi,
Okada Yasunobu
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.12.020
Subject(s) - dna laddering , dids , staurosporine , apoptosis , chemistry , channel blocker , microbiology and biotechnology , programmed cell death , pharmacology , biochemistry , biology , signal transduction , protein kinase c , organic chemistry , membrane , dna fragmentation , calcium
Apoptosis of rat cardiomyocytes induced by staurosporine is prevented by a stilbene derivative (DIDS), which is a known blocker of bothCl ‐ / HCO 3 ‐exchangers and Cl − channels. To clarify its target, staurosporine‐induced activation of caspase‐3, DNA laddering and cell death were examined in cultured rat cardiomyocytes. Removal of ambientHCO 3 ‐, which minimizes the function ofCl ‐ / HCO 3 ‐exchangers, failed to affect the preventive effect of DIDS on apoptosis. A carboxylate analog Cl − channel blocker, which does not blockCl ‐ / HCO 3 ‐exchangers, also inhibited apoptotic events. Thus, rescue by DIDS of cardiomyocytes from apoptosis is mediated by blockage of Cl − channels.