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Deletion analysis in the catalytic region of the 10–23 DNA enzyme
Author(s) -
Zaborowska Zaneta,
Schubert Steffen,
Kurreck Jens,
Erdmann Volker A.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.12.008
Subject(s) - deoxyribozyme , dna , enzyme , nucleotide , chemistry , biochemistry , rna , mutant , cytosine , cleavage (geology) , microbiology and biotechnology , biology , gene , paleontology , fracture (geology)
In this study, the functional relevance of the core nucleotides of the RNA cleaving 10–23 DNA enzyme (DNAzyme) was investigated. Systematic deletion studies revealed that DNAzymes lacking thymine at position 8 (T8) retain catalytic activity comparable to that of the wild‐type enzyme. Deletion of the adjacent cytosine at position 7 (C7) also resulted in a highly active enzyme and even the double deletion mutant C7/T8 displayed cleavage activity, although the catalytic rate under multiple turnover conditions was found to be reduced by one order of magnitude. The identification of non‐essential nucleotides in the catalytic core might help to stabilize the DNAzyme against nucleolytic degradation and to overcome problems in elucidating its three‐dimensional structure.