Premium
Common co‐lipids, in synergy, impart high gene transfer properties to transfection‐incompetent cationic lipids
Author(s) -
Mukherjee Koushik,
Sen Joyeeta,
Chaudhuri Arabinda
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.116
Subject(s) - transfection , cationic polymerization , chinese hamster ovary cell , reporter gene , cytoplasm , gene delivery , chemistry , cell , biochemistry , biology , gene , microbiology and biotechnology , gene expression , receptor , organic chemistry
Efficacious cationic transfection lipids usually need either DOPE or cholesterol as co‐lipid to deliver DNA inside the cell cytoplasm in non‐viral gene delivery. If both of these co‐lipids fail in imparting gene transfer properties, the cationic lipids are usually considered to be transfection inefficient. Herein, using both the reporter gene assay in CHO, COS‐1 and HepG2 cells and the whole cell histochemical X‐gal staining assay in representative CHO cells, we demonstrate that common co‐lipids DOPE, Cholesterol and DOPC, when act in synergy, are capable of imparting improved gene transfer properties to a novel series of cationic lipids (1–5). Contrastingly, lipids 1–5 became essentially transfection‐incompetent when used in combination with each of the pure co‐lipid components alone.