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Heparin recovers AT1 receptor and its intracellular signal transduction in cultured vascular smooth muscle cells
Author(s) -
Hashimoto Tatsuo,
Kihara Minoru,
Sato Keiko,
Imai Nozomi,
Tanaka Yutaka,
Sakai Masashi,
Tamura Kouichi,
Hirawa Nobuhito,
Toya Yoshiyuki,
Kitamura Hitoshi,
Umemura Satoshi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.093
Subject(s) - vascular smooth muscle , angiotensin ii receptor type 1 , receptor , angiotensin ii , signal transduction , microbiology and biotechnology , heparin , intracellular , desmin , biology , myocyte , medicine , endocrinology , chemistry , biochemistry , immunology , smooth muscle , vimentin , immunohistochemistry
Although vascular smooth muscle cells (VSMCs) are widely used in cardiovascular research, their phenotypic change under various culture conditions is problematic to evaluate the experimental results obtained. The levels of angiotensin (Ang) type 1/2 (AT1/AT2) receptors as well as contractile and structural proteins are degraded through culture passages. The present study demonstrated that heparin recovered Ang receptors and differentiation markers, such as desmin, SM‐22 and smooth muscle α‐actin in VSMCs at the ninth passage. Heparin also potenciated Ang II‐induced activation for ERK1/2 and p38. These results suggest a potential value of heparin‐treated VSMCs as the model for analysis of Ang‐mediated signal transduction under physiological condition.