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Regulation of tau exon 10 splicing by a double stem–loop structure in mouse intron 10
Author(s) -
Yamashita Takenari,
Tomiyama Takami,
Li Qiang,
Numata Hideharu,
Mori Hiroshi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.090
Subject(s) - rna splicing , exon , intron , alternative splicing , gene isoform , biology , minigene , mutation , microbiology and biotechnology , genetics , gene , rna
Tau exon 10 (E10) splicing is a crucial event in its developmental change of tau isoform and tauopathy. To investigate the splicing mechanism, we isolated and compared mouse tau genomic sequence with human sequence. We identified a new element in mouse intron 10 (I10) to suppress E10 splicing, which was located just after the stem–loop region previously proposed in human sequence and found to potentially form another stem–loop. Human I10 with a mutation (+29G to A) causing a decreased E10 splicing was also predicted to form similar double stem–loop, suggesting that this element is universally involved in regulation of E10 splicing.