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A dioxin sensitive gene, mammalian WAPL , is implicated in spermatogenesis
Author(s) -
Kuroda Masahiko,
Oikawa Kosuke,
Ohbayashi Tetsuya,
Yoshida Keiichi,
Yamada Kazuhiko,
Mimura Junsei,
Matsuda Yoichi,
Fujii-Kuriyama Yoshiaki,
Mukai Kiyoshi
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.070
Subject(s) - spermatogenesis , embryonic stem cell , biology , gene , microbiology and biotechnology , messenger rna , gene expression , andrology , endocrinology , genetics , medicine
2,3,7,8‐Tetrachlorodibenzo‐ p ‐dioxin (TCDD) is an endocrine disruptor that produces a variety of toxic effects. We have isolated a mouse homolog of the hWAPL gene, termed mouse WAPL ( mWAPL ), as a target of TCDD by cDNA representational difference analysis from mouse embryonic stem cells. A statistically significant increase in mWAPL expression was observed at 0.1 μM TCDD in AhR −/− mouse embryonic fibroblast cells. Interestingly, at 1 μM TCDD, mWAPL mRNA levels decreased in AhR +/+ cells, but further increased in AhR −/− cells. hWAPL and mWAPL were highly expressed only in testes among normal tissue samples, and we observed mWAPL localization in the synaptonemal complex of testicular chromosomes. In addition, mouse testes decreased the expression of mWAPL mRNA after a single intraperitoneal injection of TCDD. Thus, mammalian WAPL such as hWAPL and mWAPL may be involved in spermatogenesis and be target genes mediating the reproductive toxicity induced by TCDD.