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Overexpression of a family of RPEL proteins modifies cell shape
Author(s) -
Favot Laure,
Gillingwater Marc,
Scott Caroline,
Kemp Paul R.
Publication year - 2005
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.054
Subject(s) - rna splicing , ensembl , nuclear protein , gene , actin , gene expression , biology , cytoskeleton , alternative splicing , nucleus , microbiology and biotechnology , actin cytoskeleton , cell nucleus , cell , genetics , exon , transcription factor , rna , genome , genomics
Proteins containing RPEL motifs (e.g., MAL) are important in the regulation of gene expression by the actin cytoskeleton. Screening the ENSEMBL database for RPEL proteins identified four additional proteins that contain RPEL motifs and nuclear localisation sequences, three of which (RPEL‐A, RPEL‐B and RPEL‐C) are expressed in adult mouse tissues with different expression profiles. The mRNAs encoding RPEL‐B and RPEL‐C were subject to alternative splicing. Expression of these genes in cells indicated that they had a marked effect on cell shape. Furthermore, when expressed with a nuclear localised actin all of the different forms became restricted to the nucleus.