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Diverse human aldolase C gene promoter regions are required to direct specific LacZ expression in the hippocampus and Purkinje cells of transgenic mice
Author(s) -
Buono Pasqualina,
Barbieri Ottavia,
Alfieri Andreina,
Rosica Annamaria,
Astigiano Simonetta,
Cantatore Daniela,
Mancini Annamaria,
Fattoruso Olimpia,
Salvatore Francesco
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.032
Subject(s) - aldolase a , biology , gene expression , transgene , genetically modified mouse , microbiology and biotechnology , gene , hippocampus , fructose bisphosphate aldolase , regulation of gene expression , genetics , enzyme , biochemistry , neuroscience
Aldolase C is selectively expressed in the hippocampus and Purkinje cells in adult mammalian brain. The gene promoter regions governing cell‐specific aldolase C expression are obscure. We show that aldolase C messenger expression in the hippocampus is restricted to CA3 neurons. The human distal promoter region (−200/−1200 bp) is essential for β‐galactosidase (β‐gal) expression in CA3 neurons and drives high stripe‐like β‐gal expression in Purkinje cells. The 200 bp proximal promoter region is sufficient to drive low brain‐specific and stripe‐like β‐gal expression in Purkinje cells. Thus, the human aldolase C gene sequences studied drive endogenous‐like expression in the brain.