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Perturbation of the conformational equilibria in Ras by selective mutations as studied by 31 P NMR spectroscopy
Author(s) -
Spoerner Michael,
Wittinghofer Alfred,
Kalbitzer Hans Robert
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.020
Subject(s) - nuclear magnetic resonance spectroscopy , guanosine , chemistry , effector , spectroscopy , conformational isomerism , conformational change , crystallography , biophysics , stereochemistry , molecule , biochemistry , biology , physics , organic chemistry , quantum mechanics
Ras regulates a variety of different signal transduction pathways acting as molecular switch. It was shown by liquid and solid‐state 31 P NMR spectroscopy that Ras exists in the guanosine‐5′‐(β,γ‐imido)triphosphate bound form in at least two conformational states interconverting in millisecond time scale. The relative population between the two conformational states affects drastically the affinity of Ras to its effectors. 31 P NMR spectroscopy shows that the conformational equilibrium can be shifted specifically by point mutations, including mutations with oncogenic potential, thus modifying the effector interactions and their coupling to dynamic properties of the protein.