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Mitochondrial targeting sequence of the influenza A virus PB1‐F2 protein and its function in mitochondria
Author(s) -
Yamada Hiroshi,
Chounan Ritsu,
Higashi Youichirou,
Kurihara Naoki,
Kido Hiroshi
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.017
Subject(s) - mutagenesis , mitochondrion , vero cell , transfection , mutant , virus , biology , microbiology and biotechnology , inner mitochondrial membrane , hela , chemistry , virology , biochemistry , gene , in vitro
The influenza A virus PB1‐F2 protein predominantly localizes in the mitochondria of virus‐infected cells. A series of cDNAs encoding N‐ and C‐terminal deletion mutants and site‐directed mutagenesis of the basic residues of PB1‐F2 appended to 3xFLAG revealed the domain from residues 46 to 75 to be both necessary and sufficient for mitochondrial targeting. In addition, the subdomain residues 63–75 and both Lys73 and Arg75 are minimally required for mitochondrial localization. Transfection of untagged‐ and 3xFLAG tagged‐PB1‐F2 into Vero, HeLa and MDCK cells changed the mitochondrial morphology from a filamentous to a dotted structure and suppressed the inner‐membrane potential.