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Unique constitution of photosystem I with a novel subunit in the cyanobacterium Gloeobacter violaceus PCC 7421
Author(s) -
Inoue Hidetoshi,
Tsuchiya Tohru,
Satoh Soichirou,
Miyashita Hideaki,
Kaneko Takakazu,
Tabata Satoshi,
Tanaka Ayumi,
Mimuro Mamoru
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.013
Subject(s) - photosystem ii , protein subunit , chemistry , photosystem i , stereochemistry , biochemistry , photosynthesis , gene
Constitution of the photosystem I complex isolated from the cyanobacterium Gloeobacter violaceus PCC 7421 was investigated by tricine–urea–SDS–PAGE, followed by peptide mass fingerprinting or N‐terminal sequencing. Eight subunits (PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaL and PsaM) were identified as predicted from the genome sequence. A novel subunit (PsaZ) was discovered, but PsaI, PsaJ, PsaK and PsaX were absent. PsaB has a C‐terminal extension with 155 amino acids in addition to the conserved region and this domain is similar to the peptidoglycan‐binding domain. These results suggest that PS I complexes of G. violaceus have unique structural properties.